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cc136520 发表于 2007-12-3 18:49

IN SITU 原位反应

如题,这个概念似乎非常热门,那位吧友作些说明,扫个盲?谢谢了


百度了下,结果如下:
       指在实际反应条件下进行的反应。为了解实际反应条件下催化剂的物化性能及催化机理等信息,发展了多种原位反应的检测技术,如原位红外光谱等,以克服许多大型物理化学分析仪器原配置难以进行原位反应检测的缺点。

原位杂交;hybridization in-situ;in-situ hybridization
        (1)一种克隆筛选方法。将DNA克隆涂布或点布在固体培养基表面,形成菌管或噬菌斑后,将其转移至硝酸纤维素滤膜或尼龙膜上,与同位素(或非同位素)标记的DNA(或RNA)探针(probe)杂交,以探知含有与探针同顺序列的克隆的存在。(2)DNA片段在染色体上定位的技术。应用以同位素或荧光染料标记的DNA探针与染色体DNA杂交。在光学显微镜(或荧光显微镜)下可以看到DNA探针与相互补DNA序列结合的位置。采用不同颜色的荧光标记,可以同时测定几个DNA探针的染色体定位及它们之间的序列关系。

原位定量;in-situ quantitation
      平面色谱中,试样经展开后,组分不用转移或洗脱,直接在纸或薄层板上进行定量测定。它是利用光学扫描仪(光密度计)直接在薄层上对物质斑点进行扫描的定量方法。


xinlixi的帖子[url=http://chem8.org/bbs/viewthread.php?tid=7366&highlight=%D4%AD%CE%BB]http://chem8.org/bbs/viewthread.php?tid=7366&highlight=%D4%AD%CE%BB[/url]
给出了‘传统原位光谱用于催化表征局限性’的Rev,可惜我隔行隔山......

另外,好像还有个“ex situ 移位”,不知道而这关系如何

lfang 发表于 2007-12-3 20:34

in situ指在实际进行操作的时候同步进行表征。
ex situ指操作后或者关键的时候取出进行表征。
我也说不太明白,给你举个例子。
你做了一个电池,连接了回路,灯泡亮了。
你想了解一下电池运行的过程,灯亮的时候你研究,做表征就是in situ的,
有电流的条件下,一些表征可能无法进行,你就把电路断开,把电池拿出来,单独做表征,就是ex situ的。
不知道我说的对不对。

welai 发表于 2007-12-3 21:37

原位反应还是第一次听说.原位复合材料还知道不少
^_^:zz11)

bioprotein 发表于 2007-12-6 10:54

个人认为,在检测中,可以翻译成“实时”
在生物中,应该是“原位"不破坏分子固有的存在状态!

myheric 发表于 2007-12-6 12:41

我知道有关原位反应一般是在做催化反应中的,原位催化剂阿啥的,有点一锅法的感觉

cc136520 发表于 2007-12-20 06:41

[b][i]Chem. Soc. Rev.[/i][/b], 2008, [b]37[/b], 84 - 100, [b]DOI:[/b] 10.1039/b616738c

[size=150%][size=6][b][color=#9c0000][i]In situ[/i] hydrothermal synthesis of tetrazole coordination polymers with interesting physical properties[/color][/b]

[/size][/size][b]Hong Zhao, Zhi-Rong Qu, Heng-Yun Ye and Ren-Gen Xiong[/b]

Tetrazole compounds have been studied for more than one hundred years and applied in various areas. Several yeas ago Sharpless and his co-workers reported an environmentally friendly process for the preparation of 5-substituted 1[i]H[/i]-tetrazoles in water with zinc salt as catalysts. To reveal the exact role of the zinc salt in this reaction, a series of hydrothermal reactions aimed at trapping and characterizing the solid intermediates were investigated. This study allowed us to obtain a myriad interesting metal–organic coordination polymers that not only partially showed the role of the metal species in the synthesis of tetrazole compounds but also provided a class of complexes displaying interesting chemical and physical properties such as second harmonic generation (SHG), fluorescence, ferroelectric and dielectric behaviors. In this [i]tutorial review[/i], we will mainly focus on tetrazole coordination compounds synthesized by [i]in situ[/i] hydrothermal methods. First, we will discuss the synthesis and crystal structures of these compounds. Their various properties will be mentioned and we will show the applications of tetrazole coordination compounds in organic synthesis. Finally, we will outline some expectations in this area of chemistry. The direct coordination chemistry of tetrazoles to metal ions and [i]in situ[/i] synthesis of tetrazole through cycloaddition between organotin azide and organic cyano group will be not discussed in this review.
[attach]4840[/attach]

[url=http://www.rsc.org/Publishing/Journals/CS/article.asp?doi=b616738c]http://www.rsc.org/Publishing/Journals/CS/article.asp?doi=b616738c[/url]

wolffporter 发表于 2007-12-30 13:39

我觉得,讨论概念最好从其语义上来说,这样能更好地理解其意义。
自己的一点看法,所谓“原位”,就是指保持反应物在其初始时刻所处的区域进行反应或者被检测等活动,而不发生位移。

happybird 发表于 2008-4-3 11:12

有机上原位其实指 ipso, 比如 ipso-nitration
in situ 不好一对一的翻译,而应该根据上下文。

wangjjudy 发表于 2008-5-28 16:23

in situ 的反应现在研究的比较热门。

亚胺的形成就很多采用 in situ 的技术做它的前体化合物,该化合物在反应过程中迅速变为亚胺,

继续完成反应。

cwxa99 发表于 2008-7-7 18:49

in siu在我们这个方向  也经常遇到 我也是拿捏不准  
自己感觉可以翻译为——现场或者实时
可以到cnki翻译助手上看看
[url]http://dict.cnki.net/dict_result.aspx?tjType=all&searchword=in%20situ[/url]

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