[AnalChem]Enzyme Colorimetric Assay Using Unmodified Silver Nanoparticles
题目：[b]Enzyme Colorimetric Assay Using Unmodified Silver Nanoparticles[/b]出版：Anal. Chem., ASAP Article, 10.1021/ac801144t July 29, 2008
作者：Hui Wei, Chaogui Chen, Bingyan Han, and Erkang Wang*
摘要：Colorimetric assay based on the unique surface plasmon resonance properties of metallic nanoparticles has received considerable attention in bioassay due to its simplicity, high sensitivity, and low cost. Most of colorimetric methods previously reported employed gold nanoparticles (GNPs) as sensing elements. In this work, we develop a sensitive, selective, simple, and label-free colorimetric assay using unmodified silver nanoparticle (AgNP) probes to detect enzymatic reactions. Enzymatic reactions concerning adenosine triphosphate (ATP) dephosphorylation by calf intestine alkaline phosphatase (CIAP) and peptide phosphorylation by protein kinase A (PKA) were studied. In the absence of the enzymes, unreacted ATP could protect AgNPs from salt-induced aggregation, whereas in the presence of the enzymes, the reaction product of ATP (i.e., adenosine for CIAP and ADP for PKA) could not. Via our method, dephosphorylation and phosphorylation could be readily detected by the color change of AgNPs, with a detection limit of 1 unit/mL for CIAP and a detection limit of 0.022 unit/mL for PKA. More importantly, the enzymatic inhibition by inhibitors and enzymatic activity in complex biological fluids could also be realized. This work is an important step toward a colorimetric assay using AgNPs and might provide a promise for enzyme assay in realistically complex systems and for screening of different enzyme inhibitors in future.
链接：[url]http://pubs.acs.org/cgi-bin/abstract.cgi/ancham/asap/abs/ac801144t.html[/url] 今天刚看到，拜读完了，赞一个！！
Wei Hui兄弟你的速度真是太快了，半年过去就已经搞了三篇AC了，而且这篇文章审稿也超快啊，是直接接收的吗？ 恭祝楼主新作！ [quote]原帖由 [i]padodo[/i] 于 2008-7-29 22:25 发表 [url=http://chem8.org/bbs/redirect.php?goto=findpost&pid=67956&ptid=17879][img]http://chem8.org/bbs/images/common/back.gif[/img][/url]
今天刚看到，拜读完了，赞一个！！
Wei Hui兄弟你的速度真是太快了，半年过去就已经搞了三篇AC了，而且这篇文章审稿也超快啊，是直接接收的吗？ [/quote]
感觉我自己运气非常好，还有编辑对我们也非常友好，这篇文章是直接接收的 呵呵，再次膜拜一下wei师兄！ ：） [quote]原帖由 [i]weihui_cn[/i] 于 2008-7-29 22:35 发表 [url=http://www.chem8.org/bbs/redirect.php?goto=findpost&pid=67959&ptid=17879][img]http://www.chem8.org/bbs/images/common/back.gif[/img][/url]
感觉我自己运气非常好，还有编辑对我们也非常友好，这篇文章是直接接收的 [/quote]
冒昧的问一句，Wei兄现在是博士几年级呀？从你发的文章上看，应该够优博的条件了:D :D
另外问一句，这篇文章的编辑是哪位呀？是不是就是Yeung呀？ [quote]原帖由 [i]padodo[/i] 于 2008-7-30 09:03 发表 [url=http://chem8.org/bbs/redirect.php?goto=findpost&pid=68026&ptid=17879][img]http://chem8.org/bbs/images/common/back.gif[/img][/url]
冒昧的问一句，Wei兄现在是博士几年级呀？从你发的文章上看，应该够优博的条件了:D :D
另外问一句，这篇文章的编辑是哪位呀？是不是就是Yeung呀？ [/quote]
《Analytical Chemistry》副主编、加拿大Alberta大学化学系Richard L. McCreery教授
我，要马上毕业闪人了 Congratulations 直接接受！！！牛！ 真牛！恭喜！ 恭喜恭喜 恭喜楼主
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