Seven new prenylated indole diketopiperazine alkaloids from holothurian-derived fungus Aspergillus fumigatus
Seven new prenylated indole diketopiperazine alkaloids from holothurian-derived fungus Aspergillus fumigatusFazuo Wang, Yuchun Fanga, Tianjiao Zhua, Min Zhanga, Aiqun Lina, Qianqun Gu, Weiming Zhu
Key Laboratory of Marine Drugs, Chinese Ministry of Education, Institute of Marine Drugs and Food, Ocean University of China, Qingdao 266003, PR China
Received 18 February 2008;
revised 11 May 2008;
accepted 3 June 2008.
Available online 10 June 2008.
Abstract
Seven new prenylated indole diketopiperazine alkaloids, including compound 1, 3 spirotryprostatins C–E (2–4), 2 derivatives of fumitremorgin B (5 and 6), and 13-oxoverruculogen (7), have been isolated from the holothurian-derived fungus Aspergillus fumigatus, along with 12 known ones (8–19). The structures of the new compounds were determined on the basis of extensive spectroscopic data and amino acid analysis. All new compounds were evaluated for their cytotoxic activities on MOLT-4, A549, HL-60, and BEL-7420 cell lines by the MTT and SRB methods.
Keywords: Alkaloids; Marine-derived fungi; Aspergillus fumigatus; Structure elucidation; Cytotoxicity
Article Outline
1. Introduction
2. Results and discussion
3. Experimental
3.1. General experimental procedures
3.2. Fungal material
3.3. Fermentation, extraction, and isolation
3.3.1. Compound 1
3.3.2. Compound 2
3.3.3. Compound 3
3.3.4. Compound 4
3.3.5. Compound 5
3.3.6. Compound 6
3.3.7. Compound 7
3.4. Amino acid analysis of 1, 2, and 8
3.5. Biological assays
Acknowledgements
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