[Biosensors and Bioelectronics]--Sensitive detection of cysteine based on fluorescent silver clusters
[b]Authors[/b]: Li Shang and Shaojun Dong[b]Web Release Date[/b]:13 August 2008
[b]DOI[/b]: [u][color=#0000ff]10.1016/j.bios.2008.08.006[/color][/u]
[size=1][b]Abstract[/b][/size]: In this work, we report the application of novel, water-soluble fluorescent Ag clusters in fluorescent sensors for detecting cysteine, an important biological analyte. The fluorescence of poly(methacrylic acid) (PMAA)-templated Ag clusters was found to be quenched effectively by cysteine, but not when the other α-amino acids were present. By virtue of the specific response, a new, simple, and sensitive fluorescent method for detecting cysteine has been developed based on Ag clusters. The present assay allows for the selective determination of cysteine in the range of 2.5×10-8 M to 6.0×10-6 M with a detection limit of 20 nM at a signal-to-noise ratio of 3. Based on the absorption and fluorescence studies, we suggested that cysteine quenched the emission by the thiol-adsorption-accelerated oxidation of the emissive Ag clusters. The present study shows a promising step toward the application of silver clusters, a new class of attractive fluorescence probes.
[b]Link[/b]:[url=http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFC-4T6M81W-D&_user=3232802&_coverDate=08%2F13%2F2008&_rdoc=32&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235223%239999%23999999999%2399999%23FLA%23display%23Articles)&_cdi=5223&_sort=d&_docanchor=&view=c&_ct=195&_version=1&_urlVersion=0&_userid=3232802&md5=a7d25fd10125c09c984c8314e6deecd7]http://www.sciencedirect.com/sci ... 09c984c8314e6deecd7[/url]
[b]Remarks[/b]: 这篇工作主要是基于我前期合成的银簇（[url=http://www.chem8.org/bbs/thread-12133-1-1.html]http://www.chem8.org/bbs/thread-12133-1-1.html[/url]）的一个应用，工作主要的亮点在于发荧光的银簇这个新材料的应用的扩展。利用此银簇的另一个应用（检测铜离子）投了journal of material chemistry也已经于上月接受了，应该很快就能ASAP了。这两篇文章发表的过程还是颇费了些周折，感慨良多，待日后细说。 恭喜Sally208啊。 祝贺祝贺~
回复 1# sally208 的帖子
恭喜.同时AD一下:此帖楼主启用了自主红包发送,需要金币的chem8ers回复此帖后即可到红包中心认领,数量有限,欲抢从速.
详情:[url]http://chem8.org/bbs/plugin.php?identifier=get_money&module=money[/url]
顺便赞一下楼主的慷慨,200金币全部用来发送.:lol 祝贺胡瓜瓜 恭喜恭喜啊:) 上马甲恭喜,顺便再抢俩金币:lol Congratulation! 恭喜恭喜！！ 看看~~~做的好么 恭喜之，学习之 恭喜 恭喜! ! 恭喜恭喜~~ 恭喜恭喜阿！ 强人啊，董院士很厉害:lol 恭喜:D :D :victory: :victory: :victory: :victory: :victory: :victory: :victory: 恭喜 祝贺 可喜可贺。恭喜！