☆麦兜☆ » ASAP_Paper_Show_捷报频传 » [Talanta]Nanoparticle-amplified Surface Plasmon Resonance Study of Protein Conformational Change at Interface

fishboy 发表于 2008-9-4 11:01

[Talanta]Nanoparticle-amplified Surface Plasmon Resonance Study of Protein Conformational Change at Interface

Author: Fuan Wang, Jianlong Wang, Xiaoqing Liu and Shaojun Dong
Web Release Date: 5 July 2008.
doi:10.1016/j.talanta.2008.06.042
Abstract
This paper reports the study of protein conformational change by Au nanoparticles (AuNPs)-amplified surface plasmon resonance (SPR) spectroscopy. Taking cytochrome c (Cyt c) as an example, this paper gives a detailed description of the construction of metal–protein–metal sandwich nanostructure consisting of an Au film underlayer, a cytochrome c intermediate layer and an AuNPs upper layer. The incorporation of AuNPs into SPR biosensing results in increased SPR sensitivity to protein conformational change as demonstrated by acid denaturation of Cyt c. It suggests the conformational change of surface-confined Cyt c leads to the distance and electromagnetic coupling variations of Au film–AuNPs. The constructed Au film–Cyt c–AuNPs sandwich is stable to repeat acid treatment using solutions in the pH range of 2.0–10.0 and yields reproducible measurements. With high sensitivity and stability, nanoparticle-amplified SPR spectroscopy can be used as a supplement method to protein conformational study. It has potential for developing novel sensors and/or switching devices in response to protein conformational change.
Link: [url]http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-4SX9FVY-2&_user=1999506&_coverDate=07%2F05%2F2008&_rdoc=98&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235288%239999%23999999999%2399999%23FLA%23display%23Articles)&_cdi=5288&_sort=d&_docanchor=&_ct=183&_version=1&_urlVersion=0&_userid=1999506&md5=55e25a4c85088240e012542b9c76211c [/url]
[color=red][fbox=Random Bonus]Congratulations to fishboy, who obtained 2 token(s) from the system by posting this thread.[/fbox][/color]
Remark: 这篇文章让我极其坚强,也让我知道科研有时不仅仅是科研,其中最大的问题不是修改,而是一些人事纠纷,实验用了两个月,投稿用了近一年半.

[[i] 本帖最后由 fishboy 于 2008-9-4 11:16 编辑 [/i]]

sally208 发表于 2008-9-4 11:21

回复 1# fishboy 的帖子

很艰辛的投稿过程，还好最终能得以发表，恭喜一下！期刊也就是个载体，重要的是能发表出来。希望师兄以后科研，投稿顺利！ ：）

zjroy 发表于 2008-9-4 17:37

再接再励，发好文章

sulfur 发表于 2008-9-4 19:23

恭喜

yeqs1 发表于 2008-9-4 20:23

恭喜一下                        
:victory:

tpy60 发表于 2008-9-4 21:15

congratulation

Metalcarbene 发表于 2008-9-4 22:25

Congratulations
:zz44)

asymmsyn 发表于 2008-9-5 06:19

Congratulations!!

popeye.cn 发表于 2008-9-5 08:03

过程很复杂，但是结果很高兴。

恭喜恭喜。

padodo 发表于 2008-9-5 08:21

非常希望看到你的投稿历程，给大家分享一下吧~~~~呵呵~~

churen 发表于 2008-9-6 06:47

回复 1# fishboy 的帖子

cons!

weihui_cn 发表于 2008-9-6 09:11

:victory: :victory: :victory:

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