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fishboy 发表于 2008-9-4 11:23

[Biomacromolecules] Electrodissolution of Inorganic Ions/DNA Multilayer Film for Tunable DNA Release

Author: Fuan Wang, Dan Li, Gaiping Li, Xiaoqing Liu, and Shaojun Dong
Web Release Date: August 28, 2008
doi: 10.1021/bm800766t
Abstract
A layer-by-layer film composed of DNA and inorganic zirconium ion (Zr4+) was fabricated on the surface of gold thin film, and an electric field triggered disintegration of the multilayer film was studied by using electrochemical surface plasmon resonance (EC-SPR). EC-SPR results demonstrated that the film was disassembled upon the application of an electric field and the disassembly rate varied with the applied potential, leading to the controlled release of DNA. The electrodissolution could be switched off by removing the electric potential and reactivated by reapplying the potential. By incorporating plasmid DNA (pDNA) in to this controlled release system, the multilayer film could sustain the consecutive release of pDNA electrochemically. The released pDNA retained its integrity and transfection activity, and expressed enhanced green fluorescent protein (EGFP) after being transfected into HEK 293 cells. The electrochemical systems, with advantages of miniaturization, surface-tailoring, safety, simplicity, convenience, automation, low-cost, and free of immune reactions, made the electrical route a very attractive gene-delivery alternative.

[url]http://pubs.acs.org/cgi-bin/asap.cgi/bomaf6/asap/html/bm800766t.html[/url]
Remark: 谢谢我已经毕业的师兄.

sulfur 发表于 2008-9-4 12:03

恭喜

sally208 发表于 2008-9-4 13:33

hehe,小王师兄在这里遥谢大王师兄呢。:lol

zjniu 发表于 2008-9-4 14:21

恭喜楼主

zjroy 发表于 2008-9-4 17:36

恭喜恭喜

tpy60 发表于 2008-9-4 21:14

gxgx

Metalcarbene 发表于 2008-9-4 22:27

Congratulations
:zz44)

asymmsyn 发表于 2008-9-5 06:20

Congratulations!!!

popeye.cn 发表于 2008-9-5 08:06

谢谢分享。

zjinan 发表于 2008-9-5 08:14

祝贺！！！

padodo 发表于 2008-9-5 08:19

好文，恭喜！！

weihui_cn 发表于 2008-9-6 09:12

祝贺！！！:victory:

yeqs1 发表于 2008-9-6 13:43

恭喜楼主  
                                    
                  
:victory:

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