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Title:
Protein Sequencing and Identification Using Tandem Mass Spectrometry |
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Division: General Anal Chem / John Wiely & Son / 英文版 |
Author/Editor: Michael Kinter, Nicholas E. Sherman Star:  |
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ISBN: 0471322490 |
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Introduce Date: 2006年08月17日18:52 , Release Date: 2006年08月18日10:01 |
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Introducer: kinginsun , Rate: 11/292 |
| Format: pdf(editorial) Download |
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| Appraiser: Reuben | Grade: +5 | Reason: ( Good one! ) | |
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| Description: |
Protein Sequencing and Identification Using Tandem Mass Spectrometry
Michael Kinter, Nicholas E. Sherman
ISBN: 0-471-32249-0
Hardcover
320 pages
October 2000
Wiley List Price: US $115.00
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How to design, execute, and interpret experiments for protein sequencing using mass spectrometry
The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation.
Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes:
Peptide fragmentation and interpretation of product ion spectra
Basic polyacrylamide gel electrophoresis
Preparation of protein digests for sequencing experiments
Mass spectrometric analysis using capillary liquid chromatography
Techniques for protein identification by database searches
Characterization of modified peptides using tandem mass spectrometry
Table of Contents
An Introduction to Protein Sequencing Using Tandem Mass Spectrometry.
The Primary Structure of Proteins and a Historical Overview of Protein Sequencing.
Fundamental Mass Spectrometry.
Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra.
Basic Polyacrylamide Gel Electrophoresis.
The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments.
Mass Spectrometric Analysis of Tryptic Digests.
Protein Identification by Database Searching.
Sequence Analysis of Novel Proteins.
The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry.
Index.
Reviews
"[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." (SciTech Book News Vol. 25, No. 2 June 2001)
"...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)
"...a well-written, highly illustrative book...upper-level college students and graduate students interested in proteomics should benefit from this concise yet practical book, as would scientists making a transition into the field." (Journal of Proteome Research, Vol. 1, No. 1, January/February 2002)
"...the book does a creditable job of making detailed lab protocols accessible..." (The Analyst, September 2002)
"...very impressive by its content and its clarity..." (Biochemie, Vol.84, No.4, 2002)
http://as.wiley.com/WileyCDA/Wil ... descCd-reviews.html
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