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Title:
RNA Interference in Practice - Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals |
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Division: Genetics / John Wiley & Son / 英文版 |
Author/Editor: Ute Schepers Star:  |
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ISBN: 3527310207 |
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Introduce Date: 2006年08月24日22:54 , Release Date: 2007年12月04日08:55 |
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Introducer: leigen , Rate: 2/201 |
| Format: pdf(editorial) Download |
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| Appraiser: choscar | Grade: +5 | Reason: ( Unique! ) | |
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| Description: |
RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals
By: Ute Schepers
ISBN: 3-527-31020-7
Hardcover
336 pages
January 2005
Wiley List Price: US $135.00
Description
This hand-on guide to RNA interference makes the power of targeted gene silencing available to any laboratory with a basic equipment for the handling of nucleic acids.
In easy to follow step-by-step protocols you will learn
how RNAi works in plants, flies and mammals
how to design the most efficient RNAi constructs
how to achieve transient, stable and conditional RNAi in cell cultures
how to determine the efficiency of an RNAi experiment
how to use RNAi for gene therapy
All protocols have been thoroughly tested in the author's laboratory. Examples of successful experiments and troubleshooting hints are provided to help in establishing your own successful RNAi experiments.
Includes a list of suppliers for RNAi reagents and equipment and a glossary of terms.
Table of Contents
Preface.
1 Introduction: RNA interference, the "Breakthrough of the Year 2002".
2 RNAi in Caenorhabditis elegans.
3 RNAi in Drosophila.
4 RNAi in Mammals.
Appendix 1: Abbreviations.
Appendix 2: List of Protocols.
Appendix 3: Suppliers of RNAi-related Chemicals and Probes.
Appendix 4: Glossary of Terms.
Subject Index.
http://as.wiley.com/WileyCDA/Wil ... Cd-description.html
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